Search for: [Abstract = "In this study, we examined the effect of plant growth regulators \(2, 4\-D, 6\-BAP\) and steroidal hormone \(17\-p\-estradiol\) on a quantity of diosgenin in callus tissue of Polygonatum verticillatum \(L.\) All. The callus grown in in vitro conditions on MS medium with\; 1\/1, 2\/2, 5\/5, 10\/10 \(lmg\/dm31 2,4\-D\/lmg\/dm3\] 6\-BAP\) and 1\/1\/1,2\/2\/2, 5\/5\/5, 10\/10\/10 \(\[mg\/dm31 2,4\-D\/\(pM\/dm3j 17\-p\-estradiol\/\(mg\/dm3\) 6\-BAP\) was used for the densitometric analysis to determine the presence of diosgenin. The content of diosgenin ranged from 0,0642 to 1,1458%. An increase in diosgenin content due to addition of 6\-BAP and 2,4\-D was observed. The developed procedure was estimated by statistical analysis which indicates that it can be used for routine analysis."]

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