@misc{Oleszczuk_Sylwia_Isolated_2001,
 author={Oleszczuk, Sylwia and Zimny, Janusz},
 volume={52},
 number={1},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.52, 1 (2001)-.},
 howpublished={online},
 year={2001},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={In vitro techniques for doubled haploids (DH) production allow for obtaining homozygous lines in a single generation. This is connected with shorterbreeding cycle of the new variety. DH lines have a potential for being used inthe selection of recombinants, stabilising of transformed lines and molecularmapping. DH lines are produced from isolated microspores through haploidembryogenesis. Microspore culture has several advantages over anther culture:it reduces the time of cultures, enables monitoring of the earliest phase of embryogenesis, allows direct development of embryos, facilitates the in vitro selection and mutation, allows for avoiding regeneration from somatic anther tissues. Moreover, microspore culture appears to be a promising tool in genetic manipulations (transformation, mutagenesis) and it can be used as a source of protoplasts and suspensions},
 title={Isolated microspores as a source doubled haploids of barley},
 type={Text},
 URL={http://rcin.org.pl/Content/140018/PDF/POZN271_175474_biotechnologia-2001-no1-oleszczuk.pdf},
 keywords={biotechnology},
}