@misc{Sowa_Sławomir_In_2000,
 author={Sowa, Sławomir and Sowa, Aleksander and Zimny, Janusz},
 volume={51},
 number={4},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.51, 4 (2000)-.},
 howpublished={online},
 year={2000},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={In vitro cultures are an integral part of plant transformation process. Genetic manipulation can be performed only on a single cell level. Therefore in vitroculture and regeneration of plants from a single cell are very important for successful transformation in vitro culture of rye is more difficult to conduct than ofothers cereals. Difficulties with in vitro regeneration of rye seems to be the mainfactor limiting the development of rye transformation systems. Genetic transformation process includes three main steps; single cell transformation, selection oftransgenic cells and regeneration of plants from single cells. Efficiency ofeach of these steps can influence the result of the transformation process. Therefore optimisation of these steps is very important.},
 title={In vitro regeneration and transformation of rye (Secale cereale L.)},
 type={Text},
 URL={http://rcin.org.pl/Content/140215/PDF/POZN271_175613_biotechnologia-2000-no4-sowa.pdf},
 keywords={biotechnology},
}