@misc{Królicka_Aleksandra_Elicitation_2000, author={Królicka, Aleksandra and Łojkowska, Ewa}, volume={51}, number={4}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.51, 4 (2000)-.}, howpublished={online}, year={2000}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={Ammi visnaga (Umbelliferae) are subtropical annual plants, which contain twogroups of pharmaceutically important substances: furanochromones and piranocoumarins. In order to check the possibility of the production of secondarymetabolites, in vitro cultures of callus and cell suspension were established. Thestudy was concentrated on the induction of production of secondary metabolites by exposing callus and cell suspension cultures to abiotic elicitors: acetylsalicylic acid, jasmonic acid and suspension of silicon dioxide and biotic elicitors;autoclaved lysates ofEnterobacter sakazaki and scleroglucan. Thin layer chromatography of methanol extracts of cultures ofA. visnaga did not indicate high induction of secondary metabolites production. Treatment of the callus culturesofA. visnaga with acetylsalicylic acid or jasmonic acid induce accumulation offuranochromone - visnagin and piranocoumarin - samidin. Exposing the callusand cell suspension cultures to the suspension of silicon dioxide indicated aninduction of accumulation of furanochromone - kelolglucoside. Further research will concentrate on quantitative determination of the level of accumulatedcompounds}, title={Elicitation of secondary metabolites in in vitro culture of Ammi visnaga}, type={Text}, URL={http://rcin.org.pl/Content/140297/PDF/POZN271_175642_biotechnologia-2000-no4-krolicka.pdf}, keywords={biotechnology}, }