@misc{Grajek_Włodzimierz_Artificial_1993, author={Grajek, Włodzimierz and Lassociński, Wojciech}, volume={20}, number={1}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.20, 1 (1993)-.}, howpublished={online}, year={1993}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={Artificial seeds, consisting of somatic embryos encapsulated with protective coating, havebeen proposed as a propagation system of plant propagation. Two types of artificial seeds havebeen developed, hydrated immobUlzed in hydrogels, and desiccated. The process of somaticembriogenesls runs In some steps. At first, the embryogenlc tissue able to grow in suspensionculture must be isolated. Subsequently, this tissue should be inducted by some auxins andkinetins Introduced to the liquid medium to start the embryogenesis. The development andmaturation of embryos carry out in special media. To scale up this process the buble columnand air-lift bioreactors are used. The more frequently material to encapsulate the somaticembryos is alginate hydrogel. The main problem in production of good quality artificial seeds islow efficiency of embryos conversion to normal plant in soil.}, title={Artificial seeds for plant propagation}, type={Text}, URL={http://rcin.org.pl/Content/159761/PDF/POZN271_193906_biotechnologia-1993-no1-grajek.pdf}, keywords={biotechnology}, }