@misc{Mikuła_Anna_Cryopreservation_2008,
 author={Mikuła, Anna},
 volume={81},
 number={2},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.81, 2 (2008)-.},
 howpublished={online},
 year={2008},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={This paper presents a review of fundamental aspects of plant cryopreservation. Liquid nitrogen has several advantages over storage of vegetatively propagated material under normal low-temperature in vitro culture and could also help in preserving genetic biodiversity. Development of efficient cryopreservation protocols based on the induction of tolerance to freezing and/or desiccation is also discussed. Cold and/or preculture acclimatization leads to ultra- structural, physiological and molecular changes in cells and they are important for improving viability after cryopreservation. The application of vitrification-based procedures and ultra-fast freezing/thawing rates could be effective and reliable for wide variety of plant species/tissues and relatively genotype independent. Majority of papers demonstrate that the liquid nitrogen allows high viability rates and re-growth without a loss of biosynthetic capacity. Up to now, there has been no clear evidence of morphological, cytological or genetic alterations due to cryopreservation.},
 title={Cryopreservation as a method to facilitate presenation of life capacity of plant cells and tissues},
 type={Text},
 URL={http://rcin.org.pl/Content/73837/PDF/POZN271_96631_biotechnologia-2008-nr2-mikula.pdf},
 keywords={biotechnology},
}