@misc{Szybka-Hrynkiewicz_Przemysław_Densitometric_2004,
 author={Szybka-Hrynkiewicz, Przemysław and Janeczko, Zbigniew},
 volume={65},
 number={2},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.65, 2 (2004)-.},
 howpublished={online},
 year={2004},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={In this study, we examined the effect of plant growth regulators (2, 4-D, 6-BAP) and steroidal hormone (17-p-estradiol) on a quantity of diosgenin in callus tissue of Polygonatum verticillatum (L.) All. The callus grown in in vitro conditions on MS medium with; 1/1, 2/2, 5/5, 10/10 (lmg/dm31 2,4-D/lmg/dm3] 6-BAP) and 1/1/1,2/2/2, 5/5/5, 10/10/10 ([mg/dm31 2,4-D/(pM/dm3j 17-p-estradiol/(mg/dm3) 6-BAP) was used for the densitometric analysis to determine the presence of diosgenin. The content of diosgenin ranged from 0,0642 to 1,1458%. An increase in diosgenin content due to addition of 6-BAP and 2,4-D was observed. The developed procedure was estimated by statistical analysis which indicates that it can be used for routine analysis.},
 type={Text},
 title={Densitometric analysis of diosgenin in extracts from callus tissue of Polygonatum verticillatum (L.) All.},
 URL={http://rcin.org.pl/Content/122362/PDF/POZN271_152733_biotechnologia-2004-no2-szybka.pdf},
 keywords={biotechnology},
}