@misc{Przyborowski_Jerzy_A._In_2004,
 author={Przyborowski, Jerzy A. and Szczukowski, Stefan and Tworkowski, Józef and Stolarski, Mariusz},
 volume={65},
 number={2},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.65, 2 (2004)-.},
 howpublished={online},
 year={2004},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Sida hermaphrodita Rusby belongs to the Malvaceae family. It is a perennial plant. Biomass obtained from sida plantations may constitute excellent raw material for renewable energy production. Sida hermaphrodita Rusby reproduces generatively, but its vegetative reproduction is also possible by root-cuttings, division of the underground part into particular roots, or cuttings obtained from green above-ground shoots. Sida seeds should be sown not earlier than after one year of storage, because immediately after harvest the percentage of germinated seeds is as low as 2%, after twelve months of storage 77.5%, to drop in successive months. Moreover, due to their hardness, sida seeds require special treatment. Therefore, an attempt was made at developing a method for in vitro micropropagation of sida plants, as an alternative method of seed material production. Two kinds of explants were used in the experiment, i.e. leaf discs 8 mm in diameter and 3 mm fragments of leaf stalks. The growth regulators were added to the MS medium in combinations, i.e.: lAA -I- kinetin; lAA -P kinetin + BAP; TDZ -I- 2.4-D; 2ip + 2.4-D. The callus formed was crumbled and transferred to two kinds of medium: MS without growth regulators and MS with kinetin, NAA, GA3 and 40 g H of sucrose. The most intensive growth of callus was observed on media containing 2ip and 2,4-D, but the highest number of roots and developing shoots was obtained on MS medium without fitohor- mones.},
 type={Text},
 title={In vitro organogenesis induction in Sida hermaphrodita Rusby},
 URL={http://rcin.org.pl/Content/122377/PDF/POZN271_152758_biotechologia-2004-no2-przyborowski.pdf},
 keywords={biotechnology},
}