@misc{Mikuła_Anna_Cryopreservation_2003,
 author={Mikuła, Anna and Rybczyński, Jan J.},
 volume={62},
 number={3},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.62, 3 (2003)-.},
 howpublished={online},
 year={2003},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Cryopreservation is recognised as the most efficient method of plant genome preservation and embryogenic potential of plant in vitro cultures withnon-changed status. Because of the risk of biological degradation of tissue during freezing treatment and it various sensibility on the chilling stress, the individual treatment with cryoprotectance and parameters of the temperature decreasing is highly required.In this presentation, following subjects will be discussed: 1) description ofsuspension cultures of various gentians, 2) cell pretreatments for surviving thestress of low temperature, 3) rearrangement of cell cytoplasm because of highsucrose concentrations, 4) the role of sucrose in the development of freezingtolerance and surviving of gentaiana suspensions, 5) the effect of strongplasmolysis induced by 3 M glucose.},
 type={Text},
 title={Cryopreservation of cell suspensions of selected Gentiana},
 URL={http://rcin.org.pl/Content/133109/PDF/POZN271_167735_biotechnologia-2003-no3-mikula.pdf},
 keywords={biotechnology},
}