@misc{Wołosowska_Sylwia_B-galactosidase_2003,
 author={Wołosowska, Sylwia and Synowiecki, Józef},
 volume={61},
 number={2},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.61, 2 (2003)-.},
 howpublished={online},
 year={2003},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={The cytoplasmic p-galactosidase/p-glucosidase from hyperthermophilicarchaeon Sulfolobus shibatae has been characterized with regard to its use in lactose hydrolysis. Cell extract was purified 16-fold to a specific activity 29.5 U/mgusing ammonium sulfate precipitation, ion-exchange chromatography, and gelfiltration. Isolated enzyme exhibited optimum activity at pH 5.5 and 98°C andhad a half-life of 7 h in acetate buffer (pH 5.5) at 90°C. Cu2+, Hg2+ and Zn^+strongly inhibited the enzyme, whereas catalytic properties of other investigated cations were barely influenced. Glucose and galactose were predominantly produced from lactose. However, at the substrate concentration of 0.15 M,small amount of lactose was converted into transgalactosylation products.},
 type={Text},
 title={B-galactosidase activity of proteins extracted from Sulfolobus shibatae},
 URL={http://rcin.org.pl/Content/135754/PDF/POZN271_170596_biotechnologia-2003-no2-wolosowska.pdf},
 keywords={biotechnology},
}