@misc{Kątska-Książkiewicz_Lucyna_Long-term_2003,
 author={Kątska-Książkiewicz, Lucyna},
 volume={60},
 number={1},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.60, 1 (2003)-.},
 howpublished={online},
 year={2003},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={The ovary of mammal contains thousands of oocytes enclosed in thepreantral and early antral follicles. Since more than 99% of ovarian oocytes undergo atresia, it would be of great practical benefit if these follicles could berescued by a long-term in vitro culture leading to provide an additional source ofgametes and allowing for more efficient improvement of the reproductive potential of female. Until now, research on the isolated preantral follicles are thebest developed in rodents. In mouse, investigations have led to follicle growthand antrum formation, ovulation in vitro and even to a few live kids after in vitromaturation and fertilization of the oocytes recovered from in vitro culturedpreantral and even primordial follicles. Recently, some progress in the development of methods that allow obtaining competent oocytes and, after IVF, evenblastocysts was shown in farm animals. However, only follicles with selectedsize can be utilized for these purposes. The successful results are encouragingfor further development of methods for culture of preantral follicles in farm animals. In the article, techniques of follicle recovery, in vitro culture systems,methods for evaluation of oocyte growth, quality and competence for maturityand IVF including our impact in development of presented methods are discussed.},
 type={Text},
 title={Long-term culture preantral and early antral ovarian follicles inmammals- perspectives and recent development},
 URL={http://rcin.org.pl/Content/136965/PDF/POZN271_172167_biotechnologia-2003-no1-k%C4%85tska.pdf},
 keywords={biotechnology},
}