@misc{Salek_Anna_Teresa_Classic_2002,
 author={Salek, Anna Teresa},
 volume={56},
 number={1},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.56, 1 (2002)-.},
 howpublished={online},
 year={2002},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={eng},
 abstract={The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficientmutants of Saccharomyces cerevisiae were enucleated after treatment withcytochalasin B.Removal of nuclei from protruding sphaeroplasts was induced bycentrifugation in a Percoll density gradient. The enucleation yield (which averaged about 80%) and the quality of the cytoplasts were best when the yeast culture had been synchronized with nocodazole before the preparation. The presence of 1 mM CaCU and ATP (10 pM) in the enucleation medium prevented theformation of fragile products or aggregation. Cytoplasts could be stored for atleast 1 day without visible deterioration.},
 type={Text},
 title={Classic techniques for improvement of industrial yeast strains: Part III - A method for enucleation of Saccharomyces sp.},
 URL={http://rcin.org.pl/Content/138414/PDF/POZN271_173779_biotechnologia-2002-no2-salek.pdf},
 keywords={biotechnology},
}