@misc{Salek_Anna_Teresa_Development_2001, author={Salek, Anna Teresa}, volume={55}, number={4}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.55, 4 (2001)-.}, howpublished={online}, year={2001}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={In the Inter-LINE (IL) PCR method, oligonucleotides GF, GR and 01, whichoriginated from mammalian cells led to highly reproducible patterns of amplified template DNA, based on the consensus of LlNE-sequences. These were usedfor the genomic fingerprinting of about 80 strains of yeast, consisting of 30 species from 13 genera. The IL-PCR technique using the above primers is describedand compared to reference methods such as Arbitrarily Primed-PCR and PulseField Gel Electrophoresis (PFGE).A comparison of the two PCR variants was performed using suitable numbers of digitized PCR-fmgerprints. A database for an automated yeast identification system is proposed.}, type={Text}, title={Development of an automated yeast identification system by the application of PCR technique: a comparison of Inter-LINE PCR with reference methods}, URL={http://rcin.org.pl/Content/138601/PDF/POZN271_173951_biotechnologia-2001-no1-salek.pdf}, keywords={biotechnology}, }