@misc{Dąbrowska_Grażyna_Differential_2001, author={Dąbrowska, Grażyna}, volume={54}, number={3}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.54, 3 (2001)-.}, howpublished={online}, year={2001}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. How'ever, methods such as differential hybridization are time-consuming and require large amounts of mRNA. Recently a new approach has been successfully developed: differential display by polymerase chain reaction (DD PCR).This technique has been proven to be highly effective in identifying sequencesthat are differentially expressed in various cell types.}, type={Text}, title={Differential display - the method of searching for specifically expressed genes}, URL={http://rcin.org.pl/Content/138877/PDF/POZN271_174171_biotechnologia-2001-no3-d%C4%85browska.pdf}, keywords={biotechnology}, }