@misc{Skucińska_Barbara_Induction_2001,
 author={Skucińska, Barbara},
 volume={54},
 number={3},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.54, 3 (2001)-.},
 howpublished={online},
 year={2001},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={The effectiveness of traditional methods for inducing genetic variation hasgreatly increased with the introduction of various techniques in vitro. The newmethods of obtaining generative and somatic hybrids in vitro have resulted in agreater recombinant variation, exceeding the levels delimited hitherto by mating barriers. The potential for producing mutants has expanded due to the useof mutant somatic cells (brought in with the explant) as well as to the application of mutagens to individual cells and protoplasts, the haploid ones in particular. Two specific types of variation, i.e. somaclonal and gametoclonal variation,have proved to arise under the influence of various factors in tissue culture.However, the full application of these two types is inhibited to some extent bythe constrains on the regeneration ability of plants in culture, on the possibilityto select variants in vitro, and on the continuity of the resulting changes. Cultures in vitro also make it possible to introduce directional genetic changesthrough the application of molecular techniques.},
 type={Text},
 title={Induction of genetic variation in vitro},
 URL={http://rcin.org.pl/Content/138883/PDF/POZN271_174176_biotechnologia-2001-no3-skucinska.pdf},
 keywords={biotechnology},
}