@misc{Szyp_Iwona_Suspension_2001,
 author={Szyp, Iwona and Tretyn, Andrzej},
 volume={52},
 number={1},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.52, 1 (2001)-.},
 howpublished={online},
 year={2001},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Suspension cultures are more suitable for physiological, biochemical andmolecular investigations than callus cultures grown on solid media, because theformer provide more homogeneous system than the latter ones. A large numberof plants were found suitable for establishing cell suspension cultures. In thisstudy we describe the obtaining of cell suspension cultures derived from callusinduced from cotyledons of Pharbitis nil. Explants isolated from plants grownunder inductive and non-inductive conditions were cultured on MS basal medium containing various concentrations and combinations ofgrowth regulators.To initiate the cell suspension culture, small clumps of friable callus obtainedfrom cotyledons were suspended in liquid callusing medium. An initial inoculumdensity was 2 x lO'^cells/ml. Every 20 days the cultures were transferred to afresh medium. The cell number in suspension was determined by direct microscopic counting with haemocytometr. The cell suspension cultures containedboth single cells and small cell aggregates},
 type={Text},
 title={Suspension cultures of Pharbitis nil},
 URL={http://rcin.org.pl/Content/140015/PDF/POZN271_175471_biotechnologia-2001-no1-szyp.pdf},
 keywords={biotechnology},
}