@misc{Doroszewska_Teresa_Attempts_2000, author={Doroszewska, Teresa and Chachulska, Anna Maria}, volume={51}, number={4}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.51, 4 (2000)-.}, howpublished={online}, year={2000}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={Preliminary investigations were carried out on the transformation of highsusceptibility tobacco cultivars. The plants were transformed by being infectedwith Agrobacterium tumefaciens with binary PROK2 - derived plasmids carryinga PVY casette in the sense and antisense orientations and with a plasmid carrying the lettuce mosaic virus (LMV) coat protein gene. Kanamycin resistant plantswere obtained following transformation. PCR testing of a selected group of regenerants revealed the presence of a transgene. Disease symptoms were absentfrom 52% oftransgenic plants inoculated with PW^. This was confirmed by ELISAvalues.}, type={Text}, title={Attempts at stimulating tobacco resistance to virus diseases}, URL={http://rcin.org.pl/Content/140300/PDF/POZN271_175649_biotechnologia-2000-no4-doroszewska.pdf}, keywords={biotechnology}, }