@misc{Starzycki_Michał_Regeneration_1999, author={Starzycki, Michał and Starzycka, Eligia and Matuszczak, Marcin}, volume={44}, number={1}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.44, 1 (1999)-.}, howpublished={online}, year={1999}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={eng}, abstract={The effects of one strain Agrobacterium tumefaciens LBA 4404/pBl 121, conditions of cocultivation and growth regulators on regeneration were studied and trials of transformation wereperformed on winter oilseed rape Brassica napus L. cv. Lirajet, Valesca and Bor. Strong shootsregeneration was observed after co-cultivation without acetosyringone. Among antibiotics thatwere used in the experiments, kanamycin A and geneticin hampered whereas carbenicillin andrifampicin stimulated shoots regeneration. The presence of npt 11 gene was confirmed by PCRand Kl, K2 primers which generate a fragment of 732 bp in length.}, type={Text}, title={Regeneration of winter oiiseed rape and trials of transformation mediated by Agrobacterium tumefaciens}, URL={http://rcin.org.pl/Content/143459/PDF/POZN271_178653_biotechnologia-1999-no1-starzycki.pdf}, keywords={biotechnology}, }