@misc{Kątska_Lucyna_Czynniki_1998, author={Kątska, Lucyna}, volume={41}, number={2}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.41, 2 (1998)-.}, howpublished={online}, year={1998}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={In vitro production of bovine embryos has become a routine, increasingly available technology.Presently this technique can yield approximately 40% of blastocysts in relation to the numberof oocytes used for in vitro maturation and fertilization. However, available data indicate that invitro produced bovine embryos are more susceptible to freezing and subject to a higher incidenceof fetal loss following transfer. The comparison between in vivo (sheep oviducts) amd in vitro(co-culture with somatic cells) embryo culture conditions revealed that in vivo conditions aresuperior in terms of quality of embryos. The factors influencing quantity and quality of in vitroproduced embryos, i.e. the size of ovarian follicle for immature oocytes recovery, methods ofooc34es recovery, morphology of immature oocytes, buli effect on IVF and possible ways toovercome these limitations as well as the effect of different embryo culture systems have beendiscussed.}, type={Text}, title={Czynniki warunkujące ilość i jakość zarodków uzyskiwanych przy użyciu kompleksowej metody in vitro u bydła}, URL={http://rcin.org.pl/Content/144327/PDF/POZN271_179600_biotechnologia-1998-no2-katska.pdf}, keywords={biotechnology}, }