@misc{Paprocka_Grażyna_Wykrywanie_1997,
 author={Paprocka, Grażyna and Kęsy, Andrzej and Fitzner, Andrzej and Niedbalski, Wiesław and Płucienniczak, Grażyna},
 volume={39},
 number={4},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.39, 4 (1997)-.},
 howpublished={online},
 year={1997},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={The polymerase chain reaction (PCR) method has been applied to the detection of FMD viralRNA in samples taken during the clinical stage of FMD from calf. Total RNA was extracted withguanidinum thiocyanate-phenol-chloroform method and reversely transcribed using AMV-reversetranscriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of theVPl coding sequence. The amplified fragment of cDNA was cloned in the phagemid pBS(+). Thefirst DNA strand was sequenced and consistently an amino acid sequence was established.Comparison between VPl amino acid sequence of FMDV types C and earlier described type Owas performed.},
 type={Text},
 title={Wykrywanie pryszczycy z zastosowaniem PCR},
 URL={http://rcin.org.pl/Content/145340/PDF/POZN271_180703_biotechnologia-1997-no4-paprocka.pdf},
 keywords={biotechnology},
}