@misc{Bilecki_Wiktor_Antisense_1996,
 author={Bilecki, Wiktor and Okruszek, Andrzej and Kwinkowski, Marek and Sanak, Marek and Przewłocki, Ryszard},
 volume={35},
 number={4},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.35, 4 (1996)-.},
 howpublished={online},
 year={1996},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={eng},
 abstract={Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form (S-ODNs)targeted to iNOS mRNA were tested in RAW 264.7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-dependent manner. Maximalinhibitory effect was >90% while control S-ODNs were ineffective. Application of Lipofectin Reagent, which itself had no significant effect, greatly increased antisense activity. Decreased levelsof the target mRNA, as demonstrated by reverse transcriptase-polymerase chain reaction (RTPCR), suggest the RNase H mediated mechanism.},
 type={Text},
 title={Antisense Oligonucleotides Against Inducible Nitric Oxide Synthase Reduce NO Production in RAW 264.7 Cells},
 URL={http://rcin.org.pl/Content/146566/PDF/POZN271_182246_biotechnologia-1996-no4-bilecki.pdf},
 keywords={biotechnology},
}