@misc{Campbell_K._H._S._The_1996, author={Campbell, K. H. S. and Ritchie, W. and Loi, P. and McWhir, J. and Wilmut, I.}, volume={33}, number={2}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.33, 2 (1996)-.}, howpublished={online}, year={1996}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={eng}, abstract={Exciting new opportunities in embryo cloning have been made possible by recent studies onthe interaction of the donor nucleus with the recipient cytoplasm following embryo reconstruction. The purpose of this paper is to review information regarding the co-ordination of nuclearand cytoplasmic events during embryo reconstruction, in particular the direct and indirect effectsof maturation/meiosis/mitosis promoting factor (MPF), upon the transferred nucleus. These willbe discussed in relation to DNA replication, the maintenance of correct ploidy, the occurrenceof chromosomal abnormalities and development of reconstructed embryos. Although this reviewis primarily concerned with the reconstruction of mammalian embryos, specific examples fromamphibians will also be cited.}, type={Text}, title={The role of cell cycle co-ordination in the development of nuclear transfer reconstructed embryos}, URL={http://rcin.org.pl/Content/147103/PDF/POZN271_182727_biotechnologia-1996-no2-campbell.pdf}, keywords={biotechnology}, }