@misc{Bielecki_Stanisław_Short-chain_1996, author={Bielecki, Stanisław and Buchowiecka, Alicja}, volume={33}, number={2}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.33, 2 (1996)-.}, howpublished={online}, year={1996}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={eng}, abstract={An approach for enzymatic synthesis of oligosaccharides via transglycosylation with endo-p1,3-glucanase from Oerskovia xanthineolytica is described. Linear -p-l,3-glucan (laminarin) wasused as the donor of glycosyl residues, whereas nitrophenyl glycosides of different monosaccharides served as acceptors. The synthesis was performed in water-organic solvent environmentwith several combinations of donor/acceptor. Employing p-nitrophenyl-P-D-xylopyranoside as anacceptor in the presence of 30% acetonitrile resulted in the production of six new glycosidesshown by FAB MS to be di-, tri- and tetrasaccharides. This enzyme is therefore suitable for thesynthesis of short-chain oligosaccharides.}, type={Text}, title={Short-chain oligosaccharide synthesis by endo-B-1,3 glucanase from Oerskovia xanthineolytica}, URL={http://rcin.org.pl/Content/147112/PDF/POZN271_182766_biotechnologia-1996-no2-bielecki.pdf}, keywords={biotechnology}, }