@misc{Szklarczyk_Arkadiusz_Antisense_1995,
 author={Szklarczyk, Arkadiusz and Kaczmarek, Leszek},
 volume={28},
 number={1},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.28, 1 (1995)-.},
 howpublished={online},
 year={1995},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Antisense oligonucleotides (asODN) have recently been used to block specific gene expressionin the rodent brain. Their targets include subunits of receptors for neurotransmitters, neuropeptides and transcription factors, i.e. those proteins, whose other blockers are not known.Successful applications of the asODN require good understanding of their pharmacokinetics,mechanisms of action and side effects in the brain. Unfortunately, very little is known in thisregard. Both intraventricular and intrastructure route of administration of phosphorodiester (O- ODN) and phosphorothioate (S - ODN) ODN to the brain were effectively employed. However,doses used, even in the case of the same analog, differ up to two orders of magnitude. Sincetranslation arrest is believed to be an effective mechanism of ODN activity in the brain, mostof the authors target the ODN to the mRNA region including the translation codon, but thereare almost no studies of the target mRNA levels. The paper reviews the recent development inthis field, offering critical evaluation of the data.},
 type={Text},
 title={Antisense oligonucleotides as a tool to study gene expression in the brain},
 URL={http://rcin.org.pl/Content/148049/PDF/POZN271_183589_biotechnologia-1995-no1-szklarczyk.pdf},
 keywords={biotechnology},
}