@misc{Ptak_Agata_Micropropagation_2010, author={Ptak, Agata and Gądek, Joanna}, volume={89}, editor={Institute of Bioorganic Chemistry PAS}, editor={Committee on Biotechnology PAS}, number={2}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.89, 2 (2010)-.}, howpublished={online}, year={2010}, language={pol}, abstract={Embryogenic callus of Leucojum aestivum was transferred to solid and liquid media in bioreactor RITA® vessels. Callus growth on the liquid medium enriched with 2 and 5 qM Picloram and 0.5 qM BA was characterized by a higher multiplication index. The greatest number of somatic embryos was observed on the callus multiplied in bioreactor using 5-minute flushing with the medium. Globular embryos developed into torpedo-stage embryos and plantlets under the influence of NAA (0.5 qM) and zeatin (5 qM). Liquid medium promoted the development of L aestivum somatic embryos and plantlets.}, type={Text}, title={Micropropagation of Leucojum aestivum L. in bioraector RITA®}, URL={http://rcin.org.pl/Content/71618/PDF/POZN271_93892_biotechnologia-2010-no2-ptak.pdf}, keywords={biotechnology}, }