@misc{Górecka_Krystyna_Obtaining_2008,
 author={Górecka, Krystyna and Krzyżanowska, Dorota and Kiszczak, Waldemar and Kowalska, Urszula and Górecki, Ryszard},
 volume={81},
 number={2},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.81, 2 (2008)-.},
 howpublished={online},
 year={2008},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Experiments with anther cultures of 22 carrot cultivars were carried out to study the effect of various factors on the effectiveness of embryogenesis in these cultures. The factors included: the stage of microsporogenesis, genotype, training of donor plants and their growth conditions. A modified B5 medium (Gamborg, et al. 1968) containing 500 mg L’ glutamine, 100 mg L ’ serine, 0.1 mg L ' of 2,4D, 0.1 mg L ’ NAA, 100 g L ’ sucrose and 6.5 g L ’ agar were used to induce androgenesis. Regeneration was carried out on MS media and B5 with reduced concentration of sucrose at 20 g L ’ without aminoacids and hormones or with small amount of hormones. Substrates that were a mixture of various components, such as peat, sand, mineral wool and charcoal, were used for adaptation. Ploidy of the obtained plants was determined by cytometry method. Homozygosity of the plants was established using two isoenzymatic systems: PGl — phosphoglucose isomerase, and /\AT — aspartate aminotransferase. Anatomical studies of embryogenesis during antlier cultures were also carried out to confirm the androgenetic origin of embryos.},
 type={Text},
 title={Obtaining homozygous carrot plants with the aid of androgenesis in anther cultures},
 URL={http://rcin.org.pl/Content/73911/PDF/POZN271_96733_biotechnologia-2008-nr2-gorecka.pdf},
 keywords={biotechnology},
}