@misc{Sinkiewicz_Izabela_Suitability_2008,
 author={Sinkiewicz, Izabela and Synowiecki, Józef},
 volume={80},
 number={1},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.80, 1 (2008)-.},
 howpublished={online},
 year={2008},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={Thermus ruber is a producer of trehalose synthase, which catalyses the conversion of maltose into trehalose by intramolecular transglucosylation. The specific activity of cell-free extract of Thermus ruber cultivated on a media without saccharides was 0.016-0.028 U/mg protein and it increased up to 0.086 U/mg in the presence of 0.5?^ maltose in the culture broth. The maximum degree of maltose conversion of about 90“6 was achieved at 10% substrate concentration. The trehalose synthase does not catalyse formation of trehalose from malto- tetraose, maltohexaose and other oligosaccharides. The optimal temperature for enzyme activity was 65°C. A maximum activity of the maltose transglucosylation was performed at pH 6.5. The highest yield of trehalose synthase was attained during cultivation of bacteria at 55°C for 48 h on media composed of 0.5% peptone, 0.1% yeast extract and 0.5% maltose or starch.},
 type={Text},
 title={Suitability of Thermus ruber as a source of trehalose synthase},
 URL={http://rcin.org.pl/Content/74272/PDF/POZN271_97469_biotechnologia-2008-nr1-sienkiewicz.pdf},
 keywords={biotechnology},
}