TY - GEN N2 - The aim of the presented research was to examine the morphogenetic response of Polemonium coeruleum explants. The donor material were 10-day-old seedlings. Surface sterilized seeds were germinated on MS medium supplemented with CA3 (1 mg dnr^). Seedling explants (shoot tips, fragments of cotyledons, hipocotyls and roots) were isolated and transferred onto solidified MS medium supplemented with different types of cytokinins (BA, KN, ZEA, 2iP) at concentrations 1.0, 3.0 and 5.0 mg dm ^ in combination with NAA (0.1 mg dnr^). All explant types were characterized by callus proliferation. It was observed that calli developed on the entire surface of hipocotyl and root fragments. On the other hand, shoot tips and cotyledonary petioles formed callus tissue at the cut ends, and petioles only at abaxial ends. The growth of calli on all explant types was strongly stimulated by ZEA. Among the explants tested, only shoot tips exhibited shoot organogenesis. The highest frequency of shoot organogenesis was observed when the explants were cultured on a medium supplemented with 5.0 mg dm-3 BA (lOO^o) or 5.0 mg dnr^ ZEA (97%). The highest shoot number per explant was obtained in the presence of 5.0 mg dnr^ZEA (8.4 on average). The presence of BA or ZEA in the proliferation medium inhibited rhizogenesis and the elongation growth of shoots. However, root organogenesis was supported by KN added into the medium. L1 - http://rcin.org.pl/Content/71607/PDF/POZN271_93871_biotechnologia-2010-no2-trejgell.pdf M3 - Text PY - 2010 IS - 2 EP - 131 KW - biotechnology A1 - Trejgell, Alina A1 - Tretyn, Andrzej A2 - Institute of Bioorganic Chemistry PAS A2 - Committee on Biotechnology PAS VL - 89 SP - 125 T1 - The effect of cytokinins on morphogenetic response of Polemonium coeruleum seedlings explants UR - http://rcin.org.pl/dlibra/publication/edition/71607 ER -