Gientka, Iwona ; Błażejak, Stanisław ; Duszkiewicz-Reinhard, Wanda
Bioconversion of trans-cinnamic acid to L-phenylalanine by Rhodotorula sp
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The synthesis of the aromatic amino acid L-phenylalanine has received considerable attention in recent years due to its increasing importance as precursorto the dipeptide sweetener aspartame. Phenylalanine ammonia lyase (PAL), whichoccurs in yeast, catalyzes the nonoxidative deamination of L-Phe to trans-cinnamicacid (tCA), has industrial application in the synthesis of L-Phe. Superior producers of PAL are Rhodotorula sp. PAL is induced in yeast cell by the presence ofL-Phe, while glucose represses PAL synthesis. Different additives and conditionsduring inducing PAL: permeabilizing, reducing and stabilization agents, as wellas pH and temperature during bioconversion targeting to higher productivity ofL-Phe were discussed.
Biotechnologia, vol.73, 2 (2006)-.
0860-7796 ; oai:rcin.org.pl:87574 ; IChB B-69
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Dec 11, 2019
802
https://rcin.org.pl/publication/114203
Edition name | Date |
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Biokonwersja kwasu frans-cynamonowego do L-fenyloalaniny przez drożdże z rodzaju Rhodotorula | Oct 2, 2020 |
Dzwonek, Monika Gniewosz, Małgorzata Primik, Marek Duszkiewicz-Reinhard, Wanda
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan