Preparation of PGIP-based genetic construct for modification of strawberry genome to obtain genotypes resistant to grey mould

Korbin, Małgorzata; Majka, Jolanta; Gruchała, Agnieszka; Żurawicz, Edward

doi:0860-7796

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Opracowanie konstrukcji pBIN19-PGIP do modyfikac|i genomu truskawki dla uzyskania genotypów odpornych na szarą pleśń Korbin, MałgorzataMajka, JolantaGruchała, AgnieszkaŻurawicz, Edward

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Opracowanie konstrukcji pBIN19-PGIP do modyfikac|i genomu truskawki dla uzyskania genotypów odpornych na szarą pleśń

Preparation of PGIP-based genetic construct for modification of strawberry genome to obtain genotypes resistant to grey mould

Korbin, Małgorzata ; Majka, Jolanta ; Gruchała, Agnieszka ; Żurawicz, Edward

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

2004

biotechnologia

Polygalacturonase inhibiting protein (PGIP) cDNA was isolated using RT-PCR from fruit of apple ‘Golden Delicious’ which was previously wounded and inoculated with Botrytis cinerea. The sequence of the cDNA showed high level of homology with the published sequence of PGIP-cDNA from apple and pear (99,7 and 97,3%) and low similarity with the sequence of cDNA from tomato (51,5%). The obtained cDNA was cloned and used for the binary construct creation. The introduction of the pBIN19-PGlP construct into strawberry plant genome seems to be a promising strategy to obtain a genotype resistant to B. cinerea, causal agent of grey mould - the most economically important fungal disease of this species.

Biotechnologia, vol.65, 2 (2004)-.