Title:

Obtaining double haploid transgenic tobacco lines by the tissue culture method

Subtitle:

Obtaining double haploid transgenic tobacco lines by the tissue culture method

Creator:

Czubacka, Anna ; Doroszewska, Teresa

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2004

Subject and Keywords:

biotechnology

Abstract:

In the study on double haploid (DH) lines of tobacco, two cultivars - MN944 and BY103 transformed with a construct carrying the coat protein gene of Lettuce Mosaic Virus (LMV) CP were used. The hybrids F] from crossing transgenic line BY 103 with its non-transformed equivalent as well as transgenic MN944 line with cv. Wiślica were used for producing haploids via inducted androgenesis. Double haploids were received by regeneration from stem fragments. The course of meiosis in pollen mother cells was analysed and pollen viability was estimated.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

37

End page:

45

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open