The hairy roots of A. montana were obtained by the infection of sterile leaves with Agrobacterium rhizogenes (strain LBA 9402). The transformation was confirmed by opin and PCR analysis. In the investigation, the optimum contitions of hairy roots growth were characterized. The best results were achieved in liquid Gamborg medium which included half-strenght macro- and microelements (1/2B5) and 50 g/l sucrose. Phytochemical analysis showed that hairy roots of A. montana produced arnifolin and chlorogenic acid.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Apr 29, 2020
Apr 29, 2020
|Kultury korzeni transformowanych Arnica montana L. - izolacja metabolitów wtórnych||Apr 29, 2020|
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