Title:

Different level of point mutation and chromosomal changes among regenerants in tissue culture of Arabidopsis thaliana (L.) Heynh.

Subtitle:

Different level of point mutation and chromosomal changes among regenerants in tissue culture of Arabidopsis thaliana (L.) Heynh.

Creator:

Nabiałkowska, Danuta ; Szarejko, Iwona ; Siwińska, Dorota

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2004

Subject and Keywords:

biotechnology

Abstract:

Using the regeneration method described by Feldman and Marks (1986), the level of somaclonal variation (frequency of gene mutations and chromosomal changes) was determined in A. thaliana regenerants from root and shoot explants. The explants were regenerated via indirect organogenesis with callus stage. The level of embryo lethal mutations and changes in chromosome number were estimated in R2 generative progeny of regenerants, and confirmed in R3 generation. The frequency of R] plants carrying gene mutations was similar for all types of explants and on average it reached 8.3%, however only 47% of Ri plants showed the presence of the same embryo mutation in R2 and R3 generation. The frequency of embryo-lethal mutants, among 25 571 embryos tested, was 1.7%, while 0.08% embryos carried chlorophyll mutations. The plants with increased chromosome number were observed in the progeny of 32.2 % Rj regenerants, i.e. with eight times higher frequency than regenerants carrying gene mutations. All polyploid plants were obtained from shoot explants, while regenerants from roots carried normal diploid number of chromosomes. The observed changes in chromosome number were discussed in relation to the different content of DNA in root and shoot cells of A. thaliana.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

79

End page:

85

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open