Metadata language
Ilczuk, Agnieszka ; Witomska, Maria
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Description: Subject and Keywords: Abstract:Selected clones of a new intergeneric species Hippeastrum x chmielii Chm. grow vigorously, flower abundantly and produce flowers in clear, vivid colors. The hybrid is suitable both for cut flower production and as a pot plant. A great demand for planting the material is expected, therefore it is necessary to elaborate an efllcient micropropagation method where due to application of growth regulators the bulblets can be differentiated from different tissues. To meet this goal, the trials were undertaken to propagate this hybrid from inflorescence shoots (scapes) obtained from bulbs (clone 2/7) chilled for 3, 5 or 7 months at 4°C. Both the length of chilling and the growth regulators in the MS medium affected bulblet regeneration. On the medium without growth regulators bulblets were scarce and appeared only on shoot explants from bulbs chilled for 5 months. The presence of 2 mg dm-^ isopenthenyladenine (2iP) and 0,2 mg dm'^ a-naphtylacetic acid (NAA), as well as combination of the same auxin with 2 mg dm-3 benzyladenine (BA), stimulated bulblet formation on the explants. After 3 months of chilling, the regeneration was the poorest and the longer the chilling period was the more bulblets were produced on explants, but only in the presence of growth regulators.
Relation:Biotechnologia, vol.65, 2 (2004)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format:application/pdf ; application/pdf
Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
Projects co-financed by: Access: