Title:

The effect of concentration of mineral salts, kind of solidifying agent, cytokinin and TIBA on adventitious regeneration of apple rootstock P.59

Subtitle:

The effect of concentration of mineral salts, kind of solidifying agent, cytokinin and TIBA on adventitious regeneration of apple rootstock P.59

Creator:

Wiejacha, Katarzyna ; Orlikowska, Teresa

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2004

Subject and Keywords:

biotechnology

Abstract:

The aim of this investigation was to establish the conditions for effective regeneration of adventitious shoots on leaf explants of apple dwarf rootstock P.59. A three stage procedure was used - the induction on medium containing thidiazuron (TDZ) and a-naphtalene acetic acid (NAA) for two weeks, then the regeneration on medium supplemented with 6-benzylaminopurine (BAP) and NAA and growth of adventitious shoots on medium with low concentration of BAP and indole-3-butyric acid (IBA). The youngest leaves from proliferating shoot cultures were taken as explants for adventitious regeneration. The effect of MS salt concentration and type of solidifying agent in the induction and regeneration media, and a type of cytokinin in the induction medium was studied. It was proved that the regeneration potential was higher when 1/2 MS salts, Gelrite and TDZ was used.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

191

End page:

198

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open