Cryopreservation is recognised as the most efficient method of plant genome preservation and embryogenic potential of plant in vitro cultures withnon-changed status. Because of the risk of biological degradation of tissue during freezing treatment and it various sensibility on the chilling stress, the individual treatment with cryoprotectance and parameters of the temperature decreasing is highly required.In this presentation, following subjects will be discussed: 1) description ofsuspension cultures of various gentians, 2) cell pretreatments for surviving thestress of low temperature, 3) rearrangement of cell cytoplasm because of highsucrose concentrations, 4) the role of sucrose in the development of freezingtolerance and surviving of gentaiana suspensions, 5) the effect of strongplasmolysis induced by 3 M glucose.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Jul 21, 2020
Jul 21, 2020
|Krioprezerwacja zawiesin komórkowych wybranych gatunkówz rodzaju Gentiana||Jul 21, 2020|
Mikuła, Anna Rybczyński, Jan J.
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Rybczyński, Jan J.
Fiuk, Agnieszka Rybczyński, Jan J.
Nowak, Jacek K.