Subtitle:

Propagation of Derzsy disease virus in goose embryo fibroblasts. An application of the Celligen Plus bioreactor

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Derzsy desease is a fatal hepatitis ofyoung geese under the age of 5 weeks.The etiological agent is a goose parvovirus (GPV) which is widespread in all major goose farming countries in Europe and Asia. At present, vaccination is theonly method preventing the loss of animals. In vitro GPV replicates only in gooseembryo fibroblasts (GEF) and this way the virus is propagated in order to obtainthe material for diagnostic tests and vaccine production.In this paper, we report the application of bioreactor Celligen Plus for GEFculture and Derzsy disease virus propagation. The method is based on immobilization and culture of cells on porous glass carriers in bioreactor. Comparing tothe traditional cell culture techniques, the described method was over 12-foldmore efficient and allowed to obtain good quality virus pool for Derzsy diseasevaccine preparation.

Relation:

Biotechnologia, vol.61, 2 (2003)-.

Volume:

61

Issue:

2

Start page:

261

End page:

267

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:135753 ; IChB B-57

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open