Title:

The influence of methylene blue and methylene green on the effectiveness of photooxidation of porcine collagen

Subtitle:

The influence of methylene blue and methylene green on the effectiveness of photooxidation of porcine collagen

Creator:

Koźma, Ewa M. ; Głowacki, Andrzej ; Olczyk, Krystyna ; Wisowski, Grzegorz ; Olczyk, Paweł ; Jura-Półtorak, Agnieszka ; Nawrat, Zbigniew

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2003

Subject and Keywords:

biotechnology

Abstract:

Photooxidation is a method of tissue fixation in the construction ofbioprostheses. This process leads to the tissue protein crosslinking under lightand dye. In the present study, the influence of methylene blue and methylenegreen on the effectiveness of pulmonary valve and aorta collagen modificationwas evaluated on the basis of photooxidized tissues sensitivity to pepsin digestion. The components of tissue hydrolysates were characterized electrophoretically. The tissues photooxidized in the presence of employing dyes showed increased resistance to enzymatic degradation in comparison to the unfixed ones.However, methylene green was more effective in valve and aorta collagencrosslinking. Hydrolysates of the tissues fixed in the presence of mentioned dyedisplayed decrease in collagen a chains and their aggregates’ content.

Relation:

Biotechnologia, vol.61, 2 (2003)-.

Volume:

61

Issue:

2

Start page:

290

End page:

302

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-57

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open