Tytuł:

Expressive gene structures of proper and modified genes

Inny tytuł:

Expressive gene structures of proper and modified genes

Twórca:

Lipiński, Daniel ; Szalata, Marlena ; Kalak, Robert ; Pławski, Andrzej ; Nuc, Katarzyna ; Kala, Marta ; Juzwa, Wojciech ; Słomska, Karolina ; Gronek, Piotr ; Jura, Jacek ; Jura, Jolanta ; Smorąg, Zdzisław ; Pieńkowski, Marek ; Słomski, Ryszard

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

2003

Temat i słowa kluczowe:

biotechnology

Abstrakt:

The genetic construct WAP6xHisHGH containing the gene encoding humangrowth hormone (hGH) and WAP promoter expressed in mammary gland of animals was prepared. The 5’ end of the gene was modified by the addition of sequence encoding six histidine residues and the sequence recognized bythrombin. In this way, the growth hormone can be easily purified by affinitychromatography and cleaved with thrombin to an active form. In the next step,the genetic construct was introduced by microinjection into male pronuclei offertilized oocytes. Transgene was detected in male rabbit of FO generation(number 61). Twelve offspring of founder rabbit of generation FI indicatedtransgene sequences. The presence of growth hormone was revealed in thesamples of milk accumulated during the lactation of females of FI generation.The genetic constructs containing chain 1 and chain 2 of Feld I, and the majorallergen produced by cat (Fedlis domesticus) were prepared.

Czasopismo/Seria/cykl:

Biotechnologia, vol.60, 1 (2003)-.

Tom:

60

Zeszyt:

1

Strona pocz.:

48

Strona końc.:

73

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open