Lipiński, Daniel ; Szalata, Marlena ; Kalak, Robert ; Pławski, Andrzej ; Nuc, Katarzyna ; Kala, Marta ; Juzwa, Wojciech ; Słomska, Karolina ; Gronek, Piotr ; Jura, Jacek ; Jura, Jolanta ; Smorąg, Zdzisław ; Pieńkowski, Marek ; Słomski, Ryszard
The genetic construct WAP6xHisHGH containing the gene encoding humangrowth hormone (hGH) and WAP promoter expressed in mammary gland of animals was prepared. The 5’ end of the gene was modified by the addition of sequence encoding six histidine residues and the sequence recognized bythrombin. In this way, the growth hormone can be easily purified by affinitychromatography and cleaved with thrombin to an active form. In the next step,the genetic construct was introduced by microinjection into male pronuclei offertilized oocytes. Transgene was detected in male rabbit of FO generation(number 61). Twelve offspring of founder rabbit of generation FI indicatedtransgene sequences. The presence of growth hormone was revealed in thesamples of milk accumulated during the lactation of females of FI generation.The genetic constructs containing chain 1 and chain 2 of Feld I, and the majorallergen produced by cat (Fedlis domesticus) were prepared.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Oct 2, 2020
Aug 21, 2020
|Ekspresyjne konstrukcje genowe prawidłowych i zmutowanych genów||Oct 2, 2020|
Jura, Jacek Słomski, Ryszard Smorąg, Zdzisław Gajda, Barbara Wieczorek, Jarosław Lipiński, Daniel Kalak, Robert Juzwa, Wojciech Zeyland, Joanna
Jasiński, Adam Słomski, Ryszard Szalata, Marlena Lipiński, Daniel
Lipiński, Daniel Juzwa, Wojciech Zeyland, Joanna Słomski, Ryszard
Nuc, Katarzyna Słomski, Ryszard
Jura, Jacek Smorąg, Zdzisław Gajda, Barbara Kareta, Wiesław Kopchick, John J. Kelder, Bruce Prieto, Pedro A.
Smorąg, Zdzisław Jura, Jacek Kopchick, John J. Gajda, Barbara Skrzyszowska, Maria Różycki, Marian Pasieka, Jarosław
Jura, Jacek Smorąg, Zdzisław Kątska, Lucyna Skrzyszowska, Maria Gajda, Barbara Ryńska, Bożenna