Title:

Phage display - a new way of selecting proteins with predefined specificities

Subtitle:

Phage display - a new way of selecting proteins with predefined specificities

Creator:

Kwaśnikowski, Piotr ; Talarek, Joanna ; Czerwiński, Marcin ; Markiewicz, Wojciech T.

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2002

Subject and Keywords:

biotechnology

Abstract:

The display of diverse repertoires of proteins on the surface of filamentousbacteriophage offeres a new way of selecting proteins with predefinedspecificities. The affinity selection process can be carried out due to unique feature of the phage expression system, the direct physical linkage between genotype and phenotype of displayed proteins. Phage dysplayed libraries of proteinshave been sucessfully used in a number of applications, including selection ofmonoclonal antibodies, receptor ligands and enzyme inhibitors, cDNA expression, epitope mapping, studies on protein-protein and protein-nucleic acids interactions. The phage dislay system is also a very decent tool to perform in vitroevolution of protein properties. This approach is based on construction andscreening of reprtoires of mutagenized proteins or protein domains and affinityselection of molecules with most desirable features.

Relation:

Biotechnologia, vol.56, 1 (2002)-.

Volume:

56

Issue:

1

Start page:

35

End page:

56

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-52

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open