Title:

Subtilisin embedded in TAC fibre - properties and application

Subtitle:

Subtilisin embedded in TAC fibre - properties and application

Creator:

Trzmiel, Tadeusz ; Szczęsna-Antczak, Mirosława ; Rzyska, Małgorzata

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2001

Subject and Keywords:

biotechnology

Abstract:

The embodiment ofsubtilisin from Bacillus subtilis IBTC-3 in triacetylocellulose(TAC) fibre was studied. The fibre preparation of proteolytic activity 51.3 AmU/gwas obtained with 16.6% of yield. The properties ofsubtilisin embedded in TACfibre were investigated. The preparation showed optimal proteolytic activity atpH 9 and temperature 65-70°C. It was stable in a wider range of pH and temperature than the native subtilisin. The value for haemoglobin was 2.6 • lO'^ M.The TAC-fibre biocatalyst, in the fixed bed column, was successfully applied forhydrolysis of casein.

Relation:

Biotechnologia, vol.55, 4 (2001)-.

Volume:

55

Issue:

4

Start page:

175

End page:

188

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-50

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open