Subtitle:

Enzyme-linked immunosorbent assay (ELISA) as a method for detection of specific antibodies in animal sera

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

This paper aims to evaluate different forms of the enzyme-linkedimmunosorbent assays (ELISA) for the detection of virus-specific antibodies andfocuses on factors which may influence the diagnostic reliability of such tests.The differences between competitive and non-competitive ELISAs are described, with reference to the influence of the antigen, the conjugated antibody, and the test sample on the test results. Attention is drawn to interference, which may result in false positive or false negative test results. The use ofmonoclonal antibodies and discriminatory tests is briefly discussed. Diagnosticreliability is described for tests that are used in monitoring or eradicationprogrammes, emphasising the consequences of false negative or false positiveresults.

Relation:

Biotechnologia, vol.54, 3 (2001)-.

Volume:

54

Issue:

3

Start page:

53

End page:

70

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138854 ; IChB B-50

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open