Tytuł:

Thermostability conditions and catalytic characterization of isocitratedehydrogenase from Bacillus licheniformis

Inny tytuł:

Thermostability conditions and catalytic characterization of isocitratedehydrogenase from Bacillus licheniformis

Twórca:

Zabielski, Piotr ; Strumiło, Sławomir ; Święcicka, Izabela

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

2001

Temat i słowa kluczowe:

biotechnology

Abstrakt:

The NADP+ - dependent isocitrate dehydrogenase (IDH) from Bacilluslicheniformis was partially purified using ammonium sulphate fractionation andgel filtration on Sephadex G-200 column. The enzyme preparation had specificactivity of 1.52 U mg ’. The temperature optimum for the IDH activity was about59°C. The Arrthenius activation energy was determined to be 65.3 kj/mol below47°C, and 18.3 kJ/mol above this temperature. The IDH activity at 65°C wasmuch protected by isocitrate and magnesium, but no NADP+. Manganese ionswere more efficient activators ofthe enzyme than Mg^+. Calcium ions rather inhibited the IDH. The Km values for DL-isocitrate and NADP+ in phosphate buffer(pH 7.4) at 20°C were 85.5 and 4.9 pM, respectively: at 58°C the correspondingvalues were 181.5 and 69.3 pM.

Czasopismo/Seria/cykl:

Biotechnologia, vol.54, 3 (2001)-.

Tom:

54

Zeszyt:

3

Strona pocz.:

219

Strona końc.:

230

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; IChB B-50

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open