Subtitle:

Micropropagation of Dendrobium kingianum Bidwill orchid

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

For the development of aseptic culture, Dendrobium kingianum Bidwill orchid pseudobulbs (2.0-3.0 cm long) with one or two terminal leaves were surface sterilized with 0.1% mercuric chloride solution and transferred to initial MSmedium, supplemented with auxin lAA at 0.5 mg dnr^ and cytokinin BA at1.0 mg dm-3. New shoots, which developed after 4-6 weeks of culture at thebase of pseudobulbs or at their upper nodes, were transferred to basal MS medium suplemented with an auxin (lAA or NAA) at 0.5 and 1.0 mg drn ^ and cytokinin Kinetin or 6-benzylaminopurine (BA) at 0.5, 1.0 and 2.0 mg dm'^ in differentcombinations. The responses of orchid cultures varied according to concentrations and type of growth regulators. From tested growth regulators, BA in concentration 2.0 mg dm-3 in combination with 0.5 mg dnr^ lAA was the most effective for shoot induction. At 0.5 mg dm^ both lAA and NAA strongly stimulatedshoots multiplication in comparison to 1.0 mg dnr^. NAA-BA combinations positively influenced the shoot length. For roots induction, the NAA-Kinetin combinations were better than the remaining ones. Kinetin stimulated the increase ofthe root length.

Relation:

Biotechnologia, vol.53, 2 (2001)-.

Volume:

53

Issue:

2

Start page:

144

End page:

147

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:139322 ; IChB B-49

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open