Transfer and transgene expression
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The development oftransgenic plants strongly depends on the stable introduction of foreign nucleic acid into the plant genome. Recently, various transformation methods have been developed and successfully used for the development of transgenic plants of agricultural importance. Various vectors havebeen prepared, however the binary vector based on agrobacterial T-DNA ismost commonly used. Of vectorless gene transfer systems, particle bombardment seems to be most useful. In both approaches, stable integration of DNA isbased on random hybridization. The transformation efficiency is measured bythe level of transgene expression and it may be potentially improved by different modifications, including insertion of multiple copies of promoter into aparticular gene, insertion of procaryotic enhancer, increasing of mRNA stability,insertion of SAR/MAR sequences at tbe ends of gene, etc. Very recently it hasbeen found that the increase of histone synthesis enhances transgene expressionpossibly as a result of increased transformation efficiency. Transgenic plantsproduction also depends on the efficiency of the plant regeneration systemwhich is used. There is no universally applicable method for regeneration ofdifferent tissues from various sources, thus regeneration protocol should bemodified appropriately for each tissue and species.
Biotechnologia, vol.52, 1 (2001)-.
0860-7796 ; oai:rcin.org.pl:140006 ; IChB B-47
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 19, 2020
262
https://rcin.org.pl/publication/175464
Edition name | Date |
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Transfer i regulacja ekspresji transgenu | Oct 2, 2020 |
Sławińska, Anna Siwek, Maria
Tyczewska, Agata Bąkowska- Żywicka, Kamila
Pieczyński, Marcin Bielewicz, Dawid Dolata, Jakub Szweykowska-Kulińska, Zofia
Broniarczyk, Justyna Koczorowska, Maria Magdalena Durzyńska, Julia Warowicka, Alicja Goździcka-Józefiak, Anna