Inny tytuł:

The use of laser light for obtaining barley haploids by Hordeumbulbosum method

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

The effect of helium-neon laser with wavelength of 632 nm on seed settingin a cross of Hordeum vulgare x H. bulbosum was studied. The seeds before sowing as well as immature embryos were irradiated with laser light. Material notirradiated with laser beams constituted the control. It was shown that stimulative dose of laser beams increased the number of seeds/100 pollinated florets incomparison with the control combination. After laser treatment, the seed setting of the line HG156, and cultivars Vada and Apex was higher by 13.0, 7.9 and3.2%, respectively. The number of obtained haploids/100 cultured embryos irradiated with laser light was also higher in comparison with the control by 1.8,0.9 and 10.8%, respectively. The obtained results show that treatment with laserbeams was more effective at the first step of haploid production (seeds/100 florets) than at the next step (haploids/100 embryos cultured). From the practicalpoint of view, better results can be obtained by irradiation ofseeds before sowing than by irradiation of immature embryos. Cultivar Apex with a positive reaction on irradiation of embryos was an exception here

Czasopismo/Seria/cykl:

Biotechnologia, vol.52, 1 (2001)-.

Tom:

52

Zeszyt:

1

Strona pocz.:

143

Strona końc.:

147

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:140019 ; IChB B-47

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open