Tytuł:

Agrobacterium T-DNA in the eukaryotic cell: nuclear import and integration into the plant genome

Inny tytuł:

Agrobacterium T-DNA in the eukaryotic cell: nuclear import and integration into the plant genome

Twórca:

Ziemienowicz, Alicja

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

2000

Temat i słowa kluczowe:

biotechnology

Abstrakt:

Agrobacterium tumefaciens, a gram-negative soil bacterium, is able to transfer DNA to most plant species causing crown gall disease in dicotyledonousplants. Due to this activity Agrobacterium is widely used for plant transformation. The transferred DNA (T-DNA) that resides on a large Ti plasmid is processed within the bacterium and is exported to the plant where it is integrated intothe chromosome. DNA transfer requires plasmid encoded virulence (vir) genesas well as several chromosomal genes. In vivo studies suggested that Agrobacterium proteins are involved in T-DNA transfer and integration. We study the function of virulence proteins VirD2 and VirE2 in T-DNA nuclear import and integration using in vitro systems. We found that the T-DNA is imported into the plantcell nucleus as a complex with VirD2 and VirE2 proteins. The C-terminal NLS ofVirD2 has a piloting function in this process. Import of the T-DNA follows theclassical NLS- and importin-dependent nuclear import pathway for proteins. Forstudies of integration of T-DNA into the plant DNA an in vitro integration/ligation assay has been designed. We have found out that VirD2 is not able to ligatethe T-DNA to the plant DNA in vitro. Consequently, plant enzymes must beinvolved in this process. Indeed, we found an activity responsible for the ligation of T-DNA in extracts from tobacco BY2 suspension cultured cells and frompea axes. This activity is likely to originate from plant DNA ligase, since the T-DNAligation shows the same requirements for hydrolysis of ATP to AMP as ligationmediated by any ATP-dependent DNA ligase. This does not, however, excludethe involvement of other plant enzymes in T-DNA integration.

Czasopismo/Seria/cykl:

Biotechnologia, vol.51, 4 (2000)-.

Tom:

51

Zeszyt:

4

Strona pocz.:

24

Strona końc.:

31

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; IChB B-47

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open