In recent years a new subgroup of necrotic potato virus Y (PVY) isolates has spread in thePolish fields. To counter this infection, the PVY resistance was introduced into selected potatoand tobaeeo cultivars. Plants were transformed by agroinfection with pROK2 derived binaryplasmids, carrying an appropriate fragment of the PVY genome (patent applied for). Kanamycinresistant plants were screened by PCR for the presence of PVY cDNA inserts, positive transformants were tested for virus resistance by inoculation with sap from infected plants, followed byobservations of disease symptoms and virus accumulation analysis. Several resistant potato andtobacco clones were identified and their phenotypes were preliminarily characterised.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Nov 18, 2020
Oct 30, 2020
|Potato and tobacco cultivars transformation towards potato virus Y resistance||Nov 18, 2020|
Pałucha, Andrzej Chrzanowska, Mirosława Zagórski, Włodzimierz Hulanicka, Danuta
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.
Doroszewska, Teresa Chachulska, Anna Maria
Sadowy, Ewa Zagórski, Włodzimierz Hulanicka, Danuta