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Wykrywanie pryszczycy z zastosowaniem PCR
Subtitle:Wykrywanie pryszczycy z zastosowaniem PCR
Creator:Paprocka, Grażyna ; Kęsy, Andrzej ; Fitzner, Andrzej ; Niedbalski, Wiesław ; Płucienniczak, Grażyna
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:The polymerase chain reaction (PCR) method has been applied to the detection of FMD viralRNA in samples taken during the clinical stage of FMD from calf. Total RNA was extracted withguanidinum thiocyanate-phenol-chloroform method and reversely transcribed using AMV-reversetranscriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of theVPl coding sequence. The amplified fragment of cDNA was cloned in the phagemid pBS(+). Thefirst DNA strand was sequenced and consistently an amino acid sequence was established.Comparison between VPl amino acid sequence of FMDV types C and earlier described type Owas performed.
Relation:Biotechnologia, vol.39, 4 (1997)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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